Optimization for in vitro regeneration in four local cultivars of indica rice

doi:10.35495/ajab.2021.01.045

Abstract

Highlights

Our agriculture sector contributed to about 17% of the total country‘s Gross Domestic Product (GDP) (BBS, 2016)
Different plant organs were used as explants especially immature embryos and mature seeds (Cai et al, 2013; Azizi et al, 2015; Kumar et al, 2017; Binte Mostafiz and Wagiran, 2018), leaf base segments (Ramesh et al, 2009), anthers (Maharani et al, 2020), microspores (Shariatpanahi et al, 2006), and immature inflorescences (Kavas et al, 2008) for callus formation and plant regeneration
Callus induction in indica rice is significantly influenced by the medium components such as carbon source, amino acids and solidifying agents described in several past experiments (Binte Mostafiz and Wagiran, 2018; Repalli et al, 2019)

Summary

Introduction

Rice (Oryza sativa L.), an essential food crop belongs to the Gramineae family. It is usually grown and cultivated in the region of south and south-east Asia. Research works on successful rice regeneration from the mature embryo have been described by many scientists (Ahmad et al, 2016; Artadana et al, 2017; Binte Mostafiz and Wagiran, 2018; Ho et al, 2018; Ming et al, 2019). Some major factors such as culture medium components, explants type, Plant Growth Regulator (PGRs) treatments, solidifying agents, and culture condition etc significantly affected the callus growth, proliferation and essential steps of regeneration. (Ge et al, 2006; Feng et al, 2011; Parmar et al, 2012; Ahmad et al, 2016; Kumar et al, 2017; Binte Mostafiz and Wagiran, 2018; Repalli et al, 2019).our experiment was undertaken to establish a mature embryo based complete regeneration protocol in local indica rice Some major factors such as culture medium components, explants type, Plant Growth Regulator (PGRs) treatments, solidifying agents, and culture condition etc significantly affected the callus growth, proliferation and essential steps of regeneration. (Ge et al, 2006; Feng et al, 2011; Parmar et al, 2012; Ahmad et al, 2016; Kumar et al, 2017; Binte Mostafiz and Wagiran, 2018; Repalli et al, 2019).our experiment was undertaken to establish a mature embryo based complete regeneration protocol in local indica rice

Methods

Results

Discussion

Conclusion