Abstract
The aim of this study was to develop a productive protocol for optimum callus induction and regeneration of Thai rice variety: Nam Roo (Oryza sativa L.). Callus was induced on either MS (Murashige and Skoog) or NB (Nitsch and Nitsch) media supplemented with various concentrations of 2,4-D(2,4-dichlorophenoxyacetic acid), 0.5 mg/l NAA (α-naphthaleneacetic acid), 1 g/l L-proline, 30 g/l sucrose and 2.6 g/l phytagel. Callus from Nam Roo seed gave its maximum mean size (391.49 mm3) and mean weight (0.3412 g.) on NB medium supplemented with 1 mg/l 2, 4-D for 4 weeks. For plant regeneration the callus was cultured on either MS or NB media containing with different concentrations of BAP (6-benzylaminopurine) and phytagel, 0.5 mg/l NAA and 30 g/l sucrose. The highest regeneration frequency (%) was observed from callus grown on MS medium composed of 2 mg/l BAP in 5.2 g/l phytagel. It took 6 weeks for the callus to regenerate into a complete plant. Finally, the plantlets were transferred into the plastic pot.
Highlights
NB [7] media supplemented with various concentrations of 2,4-D (Table 1), 1 mg/l NAA, 1 g/l L-proline, 30 g/l sucrose and 2.6 g/l phytagel
The experiments were made to determine the suitable type of medium (MS or NB media) and optimal concentration of 2,4-D for callus induction for Nam Roo rice
Callus was induced on MS and NB media supplemented with 1 mg/l NAA and various concentrations of 2,4-D which were 0.5, 1, 2, 3 and 5 mg/l for 4 weeks
Summary
Rice (Oryza sativa L.) is one of the most important cereal crops which supplies food for more than half of the world’s population. Rice yield and quality are affected by pests and diseases, as well as by environmental stress. Due to the increasing world population, the value and demand for this crop steadily rise. The techniques of biotechnology are very important for developing increased productivity and quality of rice. Tissue culture has played an increasingly important role in rice improvement in recent
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