Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis.

Xueyong Ren,Liang He,Junwen Cheng,Yanbin Wang

doi:10.3390/molecules21101335

Abstract

The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP) were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 °C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014)% (n = 5), similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 104 g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC50 0.21 mg/mL), strong reducing power, ABTS radical scavenging activity (IC50 0.29 mg/mL), and hydroxyl radical scavenging activity (IC50 0.38 mg/mL). These results indicate that SJP may be useful for developing functional health products or natural antioxidant.

Highlights

Walnut (Juglans regia L.), belonging to family Juglandaceae, is originated in south-eastern Europe, Asia Minor, India, and China [1]
Semen Juglandis and the results indicated that this drug possessed significant anti-deficiency of kidney
A total of 17 experimental runs were performed with different combinations of these 3 factors

Summary

Introduction

Walnut (Juglans regia L.), belonging to family Juglandaceae, is originated in south-eastern Europe, Asia Minor, India, and China [1]. This kind of plant is a valuable agricultural goods, but its leaves, barks, stems, pericarps, fruits, flowers, and ligneous membranes are all extensively applied for various medicinal therapy in China. Semen Juglandis and the results indicated that this drug possessed significant anti-deficiency of kidney. Some low-molecular-weight, soluble compounds of Semen Juglandis may attribute to its related biological activities [5,6], such as total alkaloids, flavonoids, saponins, pheonols or other components. Some macromolecules have attracted increasing attention in using as antioxidants for dietary supplements with the function of Molecules 2016, 21, 1335; doi:10.3390/molecules21101335 www.mdpi.com/journal/molecules

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