Optimization Extraction ofCrassostrea gigasPolysaccharides and its Antioxidant Activity and Hepatoprotective Against BCG-LPS-Induced Hepatic Injury in Mice

Abstract

A active oyster polysaccharide (AOP) was extracted from oyster tissues by ultrasonic assisted extraction. The optimal extraction conditions were 20 min at pH 8, 60C with 40 W and water to solid ratio of 10, and with this method 42.73 ± 2.34 (mg/g) of AOP yield was obtained. Additionally, the antioxidant and hepatoprotective activities of AOP were investigated. The results showed that AOP exhibited strong antioxidant effects, which included scavenging activities against DPPH radicals and hydrogen oxide with IC50 values of 3.08 and 11.18 mg/mL, respectively. For hepatoprotective activities against BCG-LPS-induced hepatic injury in mice, AOP was shown to effectively improved the liver index, spleen index and thymus index, reduced the serum levels of ALT and AST. Treatment with AOP significantly increased hepatic SOD and GSH-Px activity, GSH. The levels of TNF-α and IL-1β in BCG/LPS-induced mice liver homogenates were decreased markedly by treatment with AOP. Practical Applications This work provides an alternative way for aquatic products processing enterprises to evaluate oyster polysaccharide quality and bioactivity. AOP have been shown to have potential for nutritional or pharmaceutical applications. AOP may have hepatoprotective effects against BCG-LPS -induced liver injury, which was related to its antioxidative and immunomodulatory effects.