Abstract
The extraction condition of curcumin from Curcuma longa L was optimized through four factors and three levels orthogonal experiment based on the results of single factor tests. Under the optimal conditions: theconcentrationofethanol 80%, extraction temperature 70°C, the ratio of liquid to material 20, and extraction time 3 h, a crude extract with the yield of curcumin 56.8mg/g could be obtained. The isolation and purification of curcuminoids from the crude extract was performed on high performance counter current chromatography employing an optimized solvent system n-hexane/ethyl acetate/methanol/water (2/3/3/1, v/v/v/v). From 97mg crude sample (in which the purity of curmumin was 68.56%), 67mg curmumin, 18mg demethoxycurcumin, and 9.7mg bisdemethoxycurcumin with a high-performance liquid chromatography purity of 98.26, 97.39, and 98.67%, respectively, were obtained within 70min. The antioxidant activities and cytotoxicity of purified curcumin was comparable to that of the commercial product, indicating that the biological activity of curcumin could be maintained by this method.
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