Abstract
The main objective of the present study was to isolate polyhydroxyalkanoate-producing pseudomonads from the petroleum hydrocarbon-contaminated area, investigate bioremediation potential of these bacteria and then optimize the culture conditions for maximum bacterial growth and polyhydroxyalkanoate production from crude oil. Forty-five oil-degrading pseudomonads were isolated and 15 polyhydroxyalkanoate-producing pseudomonads have been detected. Temperature = 30 °C, incubation time = 7 days, polyhydroxyalkanoates production media and 2% (V/V) crude oil were selected as the optimum conditions for maximum bacterial growth and polyhydroxyalkanoates accumulation. Finally, the produced biopolymers were characterized by gas chromatography/mass spectrometry. Under the optimal conditions, the highest bacterial growth (optical density 600 nm) and polyhydroxyalkanoates content (% W/W) were 0.35 ± 0.05 and 22.25 ± 0.13, respectively. The present study shows that unfavorable conditions such as petroleum-contaminated area can be potential resources for polyhydroxyalkanoates producers. These isolates could be used in future bioremediation of hydrocarbons while producing industrially relevant polymers as a by-product at the optimal condition.
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