Abstract
In this study, hypsizygus marmoreus was used as raw material to extract polysaccharides by hot water extraction method. Preparation technology of Selenized Polysaccharides from Hypsizygus marmoreus (SPHM) and its antioxidant activities were investigated. Chelation rate was selected as the index, effects of initial concentration of sodium selenite (mg/mL), the mass ratio of polysaccharide to sodium selenite, chelation time and pH on the chelation rate was investigated by single factor test. Response surface method was used to optimize the preparation technology of SPHM. The preliminary structure characterization of Polysaccharides from Hypsizygus marmoreus (PHM) and SPHM was performed by infrared spectroscopy and scanning electron microscopy. The antioxidant activities of SPHM were studied by the scavenging effect of 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1- (2,4,6-trinitrophenyl) Hydrazyl (DPPH) and hydroxyl radical. The results showed that the optimum preparation technology of SPHM was as follows: the initial concentration of sodium selenite was 7.95 (mg/mL), the mass ratio of polysaccharide to sodium selenite was 6.11:1 (mg/mg), chelation time was 6.89h and pH was 5.11. Under the optimum conditions, chelate rate was 10.36%. Infrared spectroscopy and scanning electron microscopy analysis showed that selenium ions successfully combined with PHM and the surface structure of PHM and SPHM was different. Compared with HPM, the antioxidant activities of SPHM enhanced.
Highlights
Se is one of the essential trace elements in human life activities
Considering the added value of H. marmoreus, this study aims to serve as a theoretical reference for the development of the emerging antioxidant food of Selenized Polysaccharides from Hypsizygus marmoreus (SPHM)
We used H. marmoreus as the raw material and the Polysaccharides from Hypsizygus marmoreus (PHM) was extracted by water extraction and alcohol sedimentation
Summary
Improves immunity and has antiaging properties (Rayman, 2000). The main two forms of Se generally present in nature are inorganic Se and organic Se. Malgorzata et al (2012) reported that organic Se compounds have the advantage of mild toxicity, easy absorption and high bioavailability compared with inorganic Se. To reduce the original toxicity of Se, we combined the Se and polysaccharide. Chelate has antiviral and antitumor properties and is absorbed and utilized by the bodies. The chelate’s biological activity is generally higher than that of polysaccharide and inorganic Se (Zhu et al, 2016). On the basis of the present condition, synthetic Se polysaccharides, such as Enteromorpha selenide (Haitao et al, 2018), Tussilago farfara L. Se (Gaiyan et al, 2016), Cucurbita moschata (Duch. ex Lam.) Duch. ex Poiret Se (Selvi et al, 2012), Punica granatum L. bark Se (Gharzouli et al, 1999), Taraxacum mongolicum Hand-Mazz selenide (Sui et al, 2004) and Lilium brownii var. viridulum Se polysaccharide (Li et al, 2010), showed that the bioactivities of Se polysaccharides, such as bacteriostatic, antioxidant and antitumor, were improved to some extent compared with unselenized polysaccharides
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