Abstract
The SNP forID 34-SNPplex is a multiplex of ancestry informative single nucleotide polymorphism markers and was originally designed for POP6™ polymer gels. Due to the higher viscosity of POP7™, a different design is necessary to resolve peaks smaller than 40 bp when this type of polymer gel is used. The single original SNaPshot™ assay was separated and redesigned into two individual multiplex SNaPshot™ assays in which rs2304925 was moved in between two SNPs with larger amplicons. Minor adjustments to PCR parameters and primer concentrations further reduced noise background in comparison with the original protocol. Validation of the assay included reproducibility (100%), sensitivity (complete DNA-profiles up to 0.5 ng) and specificity studies (no cross-species interaction). A population study ( n = 145), including 46 Belgian samples, further validated the assay for future forensic use.
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