Abstract
Purpose: To determine the effects of temperature, pressure and extraction time on oil yield obtained from Polygonatum odoratum as well as the optimum processing conditions for supercritical carbon dioxide (CO2) extraction.Methods: Supercritical CO2 extraction technology was adopted in this experiment to study the process of extraction of volatile oil from Polygonatum odoratum while gas chromatograph-mass spectrometer (GC-MS) technique was employed to analyze the chemical composition of the volatile oil. Response surface methodology (RSM) was applied to optimize supercritical CO2 process conditions.Results: The determined optimal conditions were as follows: extraction pressure, 27 MPa; extraction temperature, 50 °C; and extraction time, 97.10 min. Under these conditions, the predicted yield of Polygonatum volatile oil was 2.04 % as against an actual oil yield of 2.02 % of the seeds. GC-MS analysis showed that the extract obtained by supercritical CO2 extraction was richer in fatty acids and flavoring substances than that obtained by hydrodistillation.Conclusion: Using RSM can optimize conditions of Polygonatum volatile oil. This provides a scientific basis for further research and development of healthy foods, as well as comprehensive utilization of Polygonatum odoratum.Keywords: Polygonatum odoratum, Volatile oil, Supercritical fluid, Carbon dioxide, Extraction, Gas chromatograph-mass spectrometer
Highlights
Polygonatum, called yuzhu, waisheng, weirui, or lingdangcai in Chinese language, originated in the Southwest region of China and is well distributed in the wild
The main objective of the study was to determine the effects of temperature, pressure and extraction time on oil yield obtained from Polygonatum odoratum and to find the optimum processing condition for supercritical CO2 extraction, which could provide a foundation for further research and comprehensive utilization of Polygonatum resources
The effect of various factors on the oil yield of Polygonatum odoratum is in the following rank order of importance: extraction pressure > extraction time > extraction temperature
Summary
Polygonatum, called yuzhu, waisheng, weirui, or lingdangcai in Chinese language, originated in the Southwest region of China and is well distributed in the wild. It can be found across the southern United States [1]. Polygonatum volatile oil, called Polygonatum essential oil, is one of the major functional components of Polygonatum. It contains various kinds of active ingredients, applicable to in the clinical biopharmaceutical industry, and the cosmetic industry [5]. Volatile oils are stored in specific secretory structures and can be extracted from leaves, flowers and seeds of the plants [6]
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