Abstract
This study was aimed at optimizing process variables, assessing the phytochemical constituents and the antimicrobial activity of the extracted oil by Soxhlet extraction using the N- hexane as a solvent from Croton macrostachyus seed. The oil extracted from croton macrostachyus seed was 45.89% which is beyond the estimated value oil yield using a reaction temperature of 70 °C, 0.5 mm of particle size, 5 hours of a reaction time affects significantly and the solute to solvent ratio from 0.01 to 0.1 g/ml had no significant effect on the extraction process. The Physicochemical Properties of the extracted oil are specific gravity of 0.9, the density of 900 kg/m3 at 15 °C, the refractive index of 1.461, Acid value of 3.5 mg NaOH/g, and Free fatty acids of 1.75, are well matched the relevant international standards of the oil quality. The structural and composition of the extracted oil from Croton macrostachyus seeds were determined using FT–IR, GC-MS respectively. This result shows that the oil obtained from croton macrostachyus seeds can be used for modern medicinal purposes instead of traditional purposes.
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