Abstract
Optimization and characterization of biodiesel production from fish oil extracted from rohu (Labeo rohita) processing waste were investigated in the present study. Wet reduction method showed highest oil yield (95.2%, v/v), compared to other methods (P < 0.05). Physicochemical properties of the oils were investigated before preparing biodiesel through transesterification reaction. Various alcohols, catalysts, reaction temperature and reaction time were evaluated for optimal biodiesel conversion of oil. The maximum yield of biodiesel (95.9%) was obtained with 0.75% KOH as catalyst, 1:0.5 (v/v) oil to methanol ratio and a reaction temperature of 55 °C for 60 min. TLC, FTIR and GC analysis suggested the triglycerides in the RPW oil have completely converted into biodiesel, which contained palmitic acid methyl ester and α-linolenic acid methyl ester as the dominant esters. Physicochemical characteristics of biodiesel were evaluated and the results suggested that quality of biodiesel were feasible. DSC study demonstrated the melting temperature of biodiesel were ranged from −52.56–4.11 °C, indicated the better cold flow property. Moreover, the oxidative stability of biodiesel was improved by adding BHT at 300 mg/kg. Therefore, extracted oil from rohu processing waste could well be used as an alternative source of low-cost feedstock for biodiesel production.
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