Abstract
The extracellular polysaccharide produced by a newly isolated strain Pseudomonas fluorescens WR-1 was purified and characterized and its production was optimized using response surface methodology. The results showed that the strain WR-1 produced one kind of EPS that was composed of arabinose, glucose and uronic acid. The molecular weight of the EPS was determined to be 6.78×106Da. The preferable culture conditions for EPS production were pH 7.0, temperature 28°C for 72h with peptone and maltose as best N and C sources, respectively. The model predicted that the maximum EPS production (39.6gL−1) was appeared with maltose 48.65gL−1, Mn2+ 1118μM and Zn2+ 901μM. The EPS also showed good H2O2 scavenging activity while moderate free radical scavenging activity and reductive ability were determined. The EPS from WR-1 may be a new source of natural antioxidants with potential value for health, food and industry.
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