Optimization and Characterization Freeze Dried Fish Protein Hydrolysate Production

M.P. Gundupalli,W. Rodiahwati,Sasithorn Kongruang,Y.S. Cheng,Nicharee Wisuthiphaet,M. Sriariyanun

doi:10.1051/e3sconf/202130202007

M.P. Gundupalli, W. Rodiahwati + Show 4 more

Open Access

https://doi.org/10.1051/e3sconf/202130202007

Copy DOI

Abstract

The production of fish protein hydrolysate from underutilized fish species is attracting the industrial interest for increasing the rich protein values. The central composite design was used to optimize the degree of enzymatic hydrolysis of freeze-dried fish protein hydrolysate (FPH) production by the application of commercial Alcalase on the recovery mixed small fish protein. The effects of time, temperature, pH and enzyme concentration on the degree of hydrolysis (DH) of five strains of fish as Rastrelliger brachysoma (short-bodied mackerel), Rastrelliger kanagurta (Indian mackerel), Leiognathidae (Ponyfish), Amblygaster leiogaster (Smooth belly sardinella) and Selaroides leptolepis (yellow-stripe scad) were experimented. Result showed that the FSH production was optimized at 2.85%v/w enzyme concentration at 61 °C, pH 8.50 for 27 min with 89.42% DH. Mathematical model was proposed and validated under the optimum condition. The high proportion (46.43%) of smaller molecular weight <1 kDa was found in hydrolysate. Freeze-dried fish protein hydrolysate was produced and revealed that three predominant amino acids as glutamine, lysine and alanine. Based on amino acid compositions, the waste fish hydrolysate showed nutritional value and high potential for the applications of feed supplementation.

Highlights

The fishery industries have been substantially increasing in catching because of the continuously increasing demand of the protein derived product to 463.8 million tons by 2050 [1]
From the optimization analysis of Alcalase hydrolysis to produce fish protein hydrolysate (FPH), the suggested optimum levels of all the variables determined by the quadratic model of Composite Statistical Design (CCD) in this study to obtained 89.42% degree of hydrolysis (DH) were 2.85%v/w Alcalase concentration at pH 8.48 for 27.18 minutes of hydrolysis time as 61°C
In terms of pH and temperature, the results in this present study are in accordance with the results of study of Yuan et al, 2008 [18] showing Alcalase 2.4 L exhibited the highest values in terms of DH when optimum temperature at 60 0C, pH 8.0 with high protein yield up to 80.33%

Summary

Introduction

The fishery industries have been substantially increasing in catching because of the continuously increasing demand of the protein derived product to 463.8 million tons by 2050 [1]. The choice of using these enzymes either endoproteinase, exoproteinase or the mixture of both is the crucial factor as the main activity with specificity toward the substrate resulting in the amino acid sequence of peptides or amino acid residues that lead to different bioactive, functional and nutritional properties in the hydrolysates [9,10,11,12,13,14]. Among these hydrolyzing enzymes, Alcalase (EC 3.4.21.62) is an approved food grade endopeptidase and a highly efficient bacterial protease from Bacillus licheniformis. Comparison of amino acid content from amino acid profile of FSH was determined

Methods

Results

Conclusion