Abstract
The incorporation of a versatile and tuneable polymer–peptide network into the pores of porous silicon photonic crystals improves the selectivity of porous silicon optical biosensors to detect certain types of matrix metalloproteinase enzymes.
Highlights
There is a need for devices that can detect various biological species for the development of biotechnology and medical diagnostics
The molecular weight obtained from Gel permeation chromatography (GPC) (Mn 1⁄4 8400 g molÀ1) is in good agreement with the molecular weight estimated by NMR (Mn 1⁄4 7500 g molÀ1)
We have demonstrated a generic approach to optimize the sensing capability of porous silicon through a modular polymer conjugation strategy where the surface of the PSi was rst modi ed with an antifouling polymer, an enzyme cleavable link was added which bridged the antifouling polymer and a second sacri cial polymer that was lost upon enzyme cleavage of the peptide
Summary
There is a need for devices that can detect various biological species for the development of biotechnology and medical diagnostics. In another study by Chien et al.,[14] polymer micelles were utilised to observe a colour change upon exposure to MMPs. A further example is shown by Tanaka et al.[15] where digestion of a biodegradable polymer by phosphatase enzymes caused the release of uorescein to measure the enzymatic activity of cell lysate. A further example is shown by Tanaka et al.[15] where digestion of a biodegradable polymer by phosphatase enzymes caused the release of uorescein to measure the enzymatic activity of cell lysate All these approaches require the need for labels. Porous silicon has emerged in the last decade as a powerful platform for label free and highly sensitive optical biosensing.[16,17,18]
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