Abstract
The orthogonal experimental design was used to optimise Aspergillus niger enzyme-assisted extraction for flavonoids-enriched extract from Dicranopteris. The results indicate that the highest extraction ratio of flavonoids by Aspergillus niger enzyme-assisted extraction could arrive 13.67% using 30ml Aspergillus niger enzyme solution at 70°C,pH5.0 for 2h extraction, which was more efficient than that by without enzymes heating extraction.The flavonoids extract exhibited quite excellent inhibitions of hydroxyl radical. Meanwhile, it was found that the bioactivities appeared to be dose-dependent of flavonoids.
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