Optimisation of Xylanase–Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

Muhammad Hariadi Nawawi,Ainun Zuriyati Asa’Ari,Norazliza Sa’Ad,Paridah Md Tahir,Rosfarizan Mohamad,Wan Zuhainis Saad,Khairul Izdihar Ismail

doi:10.3390/fermentation8030119

Muhammad Hariadi Nawawi, Ainun Zuriyati Asa’Ari + Show 5 more

Open Access

https://doi.org/10.3390/fermentation8030119

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Journal: Fermentation	Publication Date: Mar 10, 2022
Citations: 7	License type: CC BY 4.0

Affiliation: Universiti Putra Malaysia

Abstract

An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 °C, pH of 8.38, inoculum size of 4% (w/v) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model’s validity. The optimised CCD model had a 1.34-fold, 159 ± 6 U/mL greater xylanase and 5.96-fold, 205 ± 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase–pectinase resulted in a ratio of 1:1.3.

Highlights

Xylanases and pectinases have a broad range of applications in biotechnology, such as in wastewater treatment, the digestion of animal feed, food processing, brewing technology, biofuels and textiles, as well as the pulp and paper industries [1–6]
The results indicated that the observed and predicted values of both responses were in close agreement, which demonstrated that the response of surface methodology (RSM) method was effective and reliable to optimise the parameters available for the production of the enzyme
The experimental data collected resulted in enhanced production of xylanase and pectinase via submerged fermentation (SmF), and the current study found that

Summary

Introduction

Xylanases and pectinases have a broad range of applications in biotechnology, such as in wastewater treatment, the digestion of animal feed, food processing, brewing technology, biofuels and textiles, as well as the pulp and paper industries [1–6]. In the pulp and paper industry, which demand that the enzymes used must be stable in the industrial process spectrum, with a wide pH and temperature range [7,8]. There are varieties of optimal parameters that need to be studied in enzymes’ biosyntheses, such as media compositions, environmental conditions and variations of the producing strains. The enzymes produced under submerged and solid-state conditions are influenced by various nutritional and fermentation parameters. These parameters must, be designed to maximise the production of the enzymes. In submerged fermentation (SmF), microorganisms are grown in suspension in a basal medium, wherein different nutrients are dissolved or suspended in several commercial media as particulate solids [9]

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