Abstract
This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology.
Highlights
Gasoline consumption has been increased tremendously due to the increase in human population that leads to an increase in energy consumption
Biobutanol produced from the biological route of acetone-butanol-ethanol (ABE) fermentation by Clostridia species has the same chemical properties with butanol produced from petrochemical route
An optimum biobutanol production was obtained at 35 ◦ C when C. acetobutylicum
Summary
Gasoline consumption has been increased tremendously due to the increase in human population that leads to an increase in energy consumption. Intense gasoline consumption may leads to an energy crisis and environmental pollution [1,2]. Current interest has shifted towards greener biofuels such as biobutanol, bioethanol, biodiesel, biohydrogen and biomethane. Biobutanol produced from the biological route of acetone-butanol-ethanol (ABE) fermentation by Clostridia species has the same chemical properties with butanol produced from petrochemical route. This biobutanol offers an alternative solution for energy security and mitigates greenhouse gases emission as it can be derived from sustainable and renewable substrates [4,5]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have