Optimisation of headspace solid-phase microextraction for the analysis of volatile phenols in wine

Abstract

Headspace solid-phase microextraction has been applied to the analysis of volatile phenols in wine. Silica fibre coated with Carbowax–divinylbenzene was found to be more efficient at extracting these compounds than other fibres such as those coated with polydimethylsiloxane, polyacrylate, carboxen–polydimethylsiloxane, and polydimethylsiloxane–divinylbenzene. Different parameters such as extraction time, temperature of the sample during the extraction, ionic strength and sample volume were optimised using a two-level factorial design expanded further to a central composite design, in order to evaluate several possibly influential and/or interacting factors. The headspace (HS)-SPME procedure developed shows adequate detection and quantitation limits, and linear ranges for correctly analysing these compounds in wine. The recoveries obtained were close to 100%, with repeatability values lower than 16%. The method was applied to a variety of white and red wines.