Abstract
HPLC analysis of dyes from ancient objects is often difficult because of the small quantity of colorant extracted; their identification, usually based on DAD, is limited to commercially available compounds. It was shown that the structural characterisation of unknown compounds could be achieved using HPLC–MS and could be finely tuned to enable both detection and characterisation. Optimisation was carried out using source parameter step-adjustment, mobile phase composition and post-column additive testing; dye characterisation trial was carried out using MS 3 pattern study. The results clearly show advantages in comparison to DAD namely improved molecular identification and better selectivity. Unfortunately, as several compounds had the same molecular mass and MS 3 fragmentation, their retention parameters were very useful for adequate structure recognition. The optimised conditions were successfully applied to the identification of components from a small sample of wool thread dyed with madder ( Rubia tinctorum) and Our Lady's bedstraw ( Galium verum).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
