Optimisation of Biomass Production and Nutritional Value of Two Marine Diatoms (Bacillariophyceae), Skeletonema costatum and Chaetoceros calcitrans.

Abstract

Simple SummaryOne of the key constraints that is associated with the production of microalgae biomass and products, is the low yields that are associated with high production costs in microalgae cultivation units. Therefore, the aim of the present work was to improve the biomass productivity of two high-value diatom species, Skeletonema costatum and Chaetoceros calcitrans. To do so, the culture medium that was supplied to the cultures was optimised in a stepwise process, regarding the nutrient’s silicate, nitrate, phosphorus, iron, and micronutrients. For both diatoms, the results that were obtained revealed a significant increase in biomass productivity as well as an improved biochemical profile regarding increased omega-3 fatty acids contents. With this work, the optimise culture media was established for each diatom, thus providing a strategy for lower production costs that were reflected in higher productivities with higher biomass quality. Ultimately this will help improve the application of S. costatum and C. calcitrans in the aquaculture and nutraceutical industries.S. costatum and C. calcitrans are two cosmopolitan high-value centric diatoms, with a rich nutritional profile. The following work optimised the culture medium of S. costatum and C. calcitrans cultures, respectively, in a stepwise process as follows: 2.4 mM and 1.2 mM of silicate, 4 mM of nitrate, 100 µM of phosphate, 20 and 80 µM iron, and 0.5 mL L−1 of micronutrients. The results that were obtained revealed an increase in biomass productivity with a 1.8- and 3.2-fold increase in biomass that was produced by S. costatum and C. calcitrans, respectively. The biochemical profile showed an increase in high-value PUFAs such as 2.6-fold and 2.3-fold increase in EPA for S. costatum and C. calcitrans, respectively, whilst a 2.6-fold increase in DHA was detected in S. costatum cultures. The present work provides the basic tools for the industrial cultivation of S. costatum and C. calcitrans with enhanced productivity as well as improved biomass quality, two factors which are highly relevant for a more effective application of these diatoms to aquaculture and nutraceutical production.