Optimal protein requirements of young Arctic charr (Salvelinus alpinus) fed practical diets

Abstract

Young Arctic charr, Salvelinus alpinus (L.), mean weight 2.56 ± 0.02 g, were fed nine isoenergetic (˜16.6 MJ digestible energy (DE) kg−1) practical diets formulated to supply digestible crude protein (DCP) at 40g kg−1 increments from 230 to 550g kg−1, for 84 days. Mean weight gain (MWG) and specific growth rate (SGR) were determined every 14 days while carcass composition was determined at the start and end of the experiment. Growth responses attained the highest values in the fish fed the diet with 350 g kg−1 DCP. Carcass moisture gain, protein gain and apparent net lipid accumulation increased as DCP levels increased to a maximum at 350 g kg−1 after which there were no differences among treatments. Total carcass lipid and lipid gain decreased as dietary DCP increased up to 470 g kg−1 with no differences thereafter. Apparent net protein accretion decreased with increasing DCP levels up to 350 g kg−1 after which there were few differences among treaments. Protein requirements were estimated by fitting MWG and SGR data to broken line regression, quadratic and saturation kinetics models. Results from these analyses suggest that dietary DCP should be provided at between 340 and 392 g kg−1 (equivalent to ˜370 and 420g kg−1 crude protein) for optimal growth of young Arctic charr reared in similar conditions.