Optimal production of cellulolytic enzymes and their location in trichoderma pseudokonigii

Abstract

AbstractThe maximum production of cellulose enzymes (FPactivity, CMcellulase and β‐glucosidase) in the culture filtrate was observed at 27°C and pH 5.0, except for β‐glucosidase, being at 6.0. The levels of extracellular enzymes in shake and stationary culture conditions was almost equal although they reached their maxima earlier in the former. The distribution of the three enzymes in extracellular, cytosol and cell debris fractions revealed most of the FPactivity and CMcellulase occurring extracellulary. More than 50% of the β‐glucosidase was present in the cell debris fraction.