Abstract
Direct detection of singlet oxygen (1O2) luminescence around 1270 nm is the golden standard of 1O2 identification. In this study, the effect of the discrimination threshold on the detection of 1O2 luminescence that generated from the photoirradiation of Rose Bengal (RB) was evaluated by using a self-developed photon-counting detection system. The obtained results show that the discrimination threshold for photon counting has a significant impact on the intensity and shape of the measured 1O2 luminescence, which resulted in the variation of 1O2 lifetimes. The optimal discrimination threshold is determined to be about −0.0412 V, and the corresponding 1O2 lifetime in air-saturated distilled water is 4.26±0.06 μs.
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