Abstract
In this study, we discuss diffusion based oxygen and glucose supplies in cell culture chips of three different setups, where cells are regularly arranged and cultivated, using oxygen and glucose transport models on the basis of the previous work. In the first setup, oxygen is continuously supplied through a gas-permeable wall equipped with the chip, but glucose is supplied only at the start. In this case, the life span of the cultivated cells is governed by the balance between the initial amount and the consumption rate of glucose. Secondly, a setup, where oxygen and glucose are continuously supplied through a semi-permeable membrane, is discussed. In this case, oxygen supply is so critical that the membrane must be well designed. Finally, a setup with reserves for glucose supply, where oxygen is supplied through a gas-permeable wall, is discussed. In this case, we can find an optimal thickness of the medium filled in the cell culture chamber because an increase in the thickness is advantageous to glucose supply but is contrary disadvantageous to oxygen supply. In all cases, cautious design of a cell chip is needed, if the consumption rates of the cultivated cells for oxygen and glucose are higher than 10 g m −3 s −1, which are very likely for hepatocytes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.