Optimal conditions of LDR to protect the kidney from diabetes: Exposure to 12.5 mGy X-rays for 8 weeks efficiently protects the kidney from diabetes

Abstract

AimsWe reported the attenuation of diabetes-induced renal dysfunction by exposure to multiple low-dose radiation (LDR) at 25mGy every other day by suppressing renal oxidative damage. We here explored the optimal conditions of LDR to protect the kidney from diabetes. Main methodsMale C57BL/6J mice with type 1 diabetes were induced with multiple injections of low-dose streptozotocin. Diabetic mice received whole body X-irradiation at a dose of 12.5, 25 or 50mGy every other day for either 4 or 8weeks. Age-matched normal mice were similarly irradiated at the dose of 25mGy for 4 or 8weeks. The renal function and histopathological changes were examined at the 4th and 8th weeks of the study. Key findingsDiabetes induced renal dysfunction is shown by the decreased creatinine and increased microalbumin in the urine. Renal oxidative damage, detected by protein nitration and lipid oxidation, and remodeling, reflected by increased expression of connective tissue growth factor, collagen IV and fibronectin, were significantly increased in diabetic mice. All these renal pathological and function changes in diabetic mice were significantly attenuated by exposure to LDR at all regimens, among which, however, exposure to LDR at 12.5mGy for 8weeks provided the best protective effect on the kidney of diabetic mice. SignificanceOur results suggest that whole-body LDR at 12.5mGy every other day for 8weeks is the optimal condition of LDR to protect the kidney from diabetes.