Abstract
The optimal conditions for the release of protoplasts from 18-h-hyphae of Neurospora crassa using Novozym 234 were 120-minute incubations at 25°C and 10 mg/ml Novozym. Protoplasts were released from all parts of hyphae and were found not to contain surface-bound cell wall material. About 10% of Novozym-derived protoplasts were viable.
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