Optically Amplified RNA–Protein Detection Methods Using Light‐Harvesting Conjugated Polymers

Abstract

A sensor is described that takes advantage of highly specific RNA–protein interactions and the light‐harvesting properties of conjugated polymers. Coordination between a cationic conjugated polymer (CP+) and a negatively charged protein–RNA complex results in efficient Förster resonance energy transfer (FRET) from CP+ to a protein‐bound chromophore (see Figure and inside cover). This sensor was used to detect the transactivation responsive RNA sequence of HIV‐1.