Abstract
Cell manipulation is one of the most impactful applications for optical tweezers, and derived from this promise, we demonstrate a new optical tweezers system for the study of cell adhesion and organization. This method utilizes photonic-crystal-enhanced optical tweezers to manipulate cells with low laser intensities. By doing so, it enables effective cell patterning and culturing within the conditions necessary for successful differentiation and colony formation of human pluripotent stem cells. To this end, the biocompatibility of plasma-treated parylene-C for cell culturing was studied, and a thorough characterization of cellular interactive forces was performed using this system. Furthermore, this study also demonstrates construction of patterned cell arrays at arbitrary positions with micrometer-scale precision.
Highlights
Cellular spatial patterning and geometrical confinement are postulated as important factors in tissue development and disease [1, 2]
Cell manipulation is one of the most impactful applications for optical tweezers, and derived from this promise, we demonstrate a new optical tweezers system for the study of cell adhesion and organization
This study demonstrates construction of patterned cell arrays at arbitrary positions with micrometer-scale precision
Summary
Cellular spatial patterning and geometrical confinement are postulated as important factors in tissue development and disease [1, 2] To study these factors, cell cultures can provide a useful model system; we are still limited in our ability to position cells with specific geometries in culture, at the single-cell level. There are many technologies developed to measure the attachment or adhesive forces of cells, including atomic force microscope (AFM), magnetic tweezers, culture force monitor (CFM), and so on [7,8,9,10,11,12,13] These methods are used to characterize single ligand-receptor systems through their energy landscape and kinetic parameters. These methods are not compatible with either repulsive force measurement or cell patterning at the single-cell level
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
