Optical spectroscopic diagnosis of liver cancerous and normal blood serum

Abstract

Laser induced human serum Raman spectra of liver cancer are measured. The spectra differences in serum from normal people and liver cancerous people are analyzed. There is obvious difference between the spectrum of liver cancer and that of normal people. For the typical spectrum of normal serum there are three sharp Raman peaks (A at approximately 1010 cm^-1, at approximately 1160 cm^-1, C at approximately 1525 cm^-1) and relative intensity of Raman peak excited by 514.5 nm is higher than that excited by 488.0 nm. However, for the Raman spectrum of liver cancerous serum there are no peaks or very weak Raman peaks at the same positions of spectrum and intensity of Raman peak excited by 514.5 nm is lower than that excited by 488.0 nm. Results of more than two hundred case measurements show that clinical diagnostic accuracy is 92.86%. And then, the liver fibrosis is studied applying the technology of LIF. The experiment indicates that there is notable fluorescence difference between the abnormal and normal liver tissue, there is blue shift abnormal tissue in compare with normal liver tissue. These results have important reference values to explore the method of laser spectrum diagnosis.