Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells

Abstract

ABSTRACT O ptical spectral analysis of the ultra -weak photon emission (UPE) could be utilized for non -invasive diagnostic of state of biological systems and for elucidat ion of underlying mechanisms of UPE generation. Optical spectra of UPE from differentiated HL -60 cells and yeast cells (Saccharomyces cerevisiae ) were investigated . Induced photon emission of neutrophil -like cells and spontaneous photon emission of yeast cells were measured using highly sensitive photomultiplier module Hamamatsu H7360 -01 in a thermally regulated light -tight chamber. The respiratory burst of neutrophil -like HL -60 cells was induced with the PMA (phorbol 12 -myristate, 13 -acetate). PMA activates an assembly of N ADPH oxidase, which induces a rapid formation of reactive oxygen species (ROS ). Long -pass edge filters (wavelength 350, from 400 to 600 with 25 nm resolution and 650 nm) were used for optical spectral analysis. P ropagation of error of indirect measurements and standard deviation were used to assess reliability of the measured spectra. Results indicate that the photon emission from both cell cultures is detectable in the six from eight examined wavelength ranges with different percentage distribution of cell suspensions, particularly 450 -475, 475 -500, 500 -525, 525 -550, 550 -575 and 575 -600 nm. The wavelength range of spectra from 450 to 550 nm coincides with the range of photon emission from triplet excited carbonyls (350 -550 nm). The both cell s culture s emitted photons in wavelength range from 550 to 600 nm but this range does not correspond with any known emitter. To summarize, we have demonstrated a clear difference in the UPE spectra between two organisms using rigorous methodology and error analysis . Keywords: ultra-weak photon emission, optical spectrum , HL60 cells, yeast cells photomultiplier