Optical scattering serves as a prognostic biomarker for immune-mediated chemotherapy treatment response and resistance in a murine breast cancer model

Abstract

Spatial Frequency Domain Imaging (SFDI) can provide longitudinal label-free, wide field hemodynamic and scattering information of murine tumors. Here we present a longitudinal study utilizing SFDI to monitor a paired immune responsive-resistant model for up to 30 days of treatment. Mice receiving the immunomodulatory treatment had a large increase in the reduced optical scattering throughout treatment compared to the mice receiving an immune-blocking antibody. These results indicate that scattering is sensitive to the immune-mediated apoptosis of tumor cells and capable of discriminating between responsive and resistant tumor models.