Abstract
PHOSPHOLIPIDS are primary structural materials of the mitochondrial membrane, and numerous observations implicate them in the function of cytochrome c (ref. 1). Thus, when bound in the mitochondrial membrane, cytochrome c is not easily extractable and permits higher rates of electron transport than does added cytochrome c (refs. 2–4). Moreover, when the beef heart mitochondrial electron transport particle is treated with phospholipase, cytochrome c is released from the binding site as the soluble protein5. In addition, phospholipids are required for activity of purified preparations of reduced cytochrome c oxidase6–8, and for reactivation of succinoxidase activity after solvent extraction of mitochondria9.
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