Abstract
A method is described for using and calibrating the Keston polarimetric attachment to the Beckman DU spectrophotometer which dispenses with the need to reset, at each wavelength, the symmetry of the analyzer positions about total extinction with the polarizer. The instrument is calibrated with sucrose solutions of concentrations from 2.5 to 40%, over a wave-length range of 250 to 675 nm. The constancy of calibration with a maximum error of 6.5% over this range is demonstrated. The smooth but slight dependency of the calibration constant on wavelength is shown and attributed to the optical properties of the Nicol prisms. Linear regression of the data allows for a high degree of accuracy in measuring the rotation of a sample, as a function of wavelength.
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