Abstract
An advanced method is described for the complete separation of phenylthiohydantoin (PTH)- dl-amino acids for protein sequencing. Optical resolution of all standard PTH- dl-amino acids was successfully developed using some chiral selectors, although the resolution of only PTH- dl-His and Lys could not be confirmed, due to low reproducibility and the presence of impurities. In addition, mixed chiral selectors for making a single electrolyte with the ability to optically resolve all standard PTH- dl-amino acids were investigated. Using the only resulting electrolyte, sequence determination of [ d-Ala 2]-methionine enkephalin, with dl differentiation, was performed.
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