Abstract
The enantiometric resolution of amino acids, amino acid derivatives, peptides and hydroxycarboxylic acids was investigated using a chiral column of the ligand- exchange type. It was found that this column separated all of these compounds satisfactorily. Columns containing either a d- or l-ligand gave almost identical chromatograms, except that the retention order for a pair of chiral isomers was reversed. These columns could be applied to resolve simultaneously ten different types of dL- amino acids. Optically active impurities present in d and l-amino acids could be determined at concentrations down to about 100 ppm.
Published Version
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