Abstract
It has been successfully carried out the synthesis of silver nanoparticles by chemical reduction method. Silver nitrate (AgNO3) is used as the metal precursor and trisodium citrate as the reducing agent as well as the use of polyvinyl alcohol (PVA) as a stabilizer. The formation of silver nanoparticles was observed visually with discoloration (yellow). To excite surface plasmons, attenuated total reflection (ATR) method is used with Krestchmann configuration of the prism coupling. The maximum absorption band in the UV-Vis spectrometer shows a red shift of 429.43 nm wavelength for a colloidal solution of silver nanoparticles without PVA and 429.01 nm with PVA. The addition of PVA sharpened absorption spectrum curve and produce a broad absorption band which is indicative of a smaller particle size. TEM images showed that the morphology (crystallites) silver nanoparticles have nearly spherical geometry with dispersive particle distribution. Dispersibility of nanoparticles such as this could potentially be used as an active ingredient of SPR biosensor. The observation of the SPR phenomenon shows the SPR angle shift of 0.1° when a thin layer of silver as an active ingredient a biosensor coated with silver nanoparticles and 0.2° when silver nanoparticles with PVA. SPR angle shift and increase the reflectance values caused by changes in surface Plasmon, which can be a reference that the SPR phenomenon with the sensing surface modification using an additional layer of silver nanoparticles can increase sensitivity.
Highlights
Biosensor based surface plasmon resonance (SPR) is a type of optical sensor that utilizes surface plasmon polariton (SPP) waves
Optical absorption spectra of silver nanoparticles is dominated by surface plasmon which shows a shift towards the red end or blue end depends on particle size, shape, state of aggregation and the surrounding dielectric medium
The addition of polyvinyl alcohol (PVA) sharpen absorption spectrum curve and produce a broad absorption band which is indicative of a smaller particle size
Summary
Biosensor based surface plasmon resonance (SPR) is a type of optical sensor that utilizes surface plasmon polariton (SPP) waves. In recent years, the noble metal nanoparticles have been the subject of research interest because of its unique properties, such as the electronic properties, optical, mechanical, magnetic and chemical properties are significantly different from the bulk material [9]-[11]. For this reason the noble metal nanoparticles have been widely used in many applications in different fields such as catalysis, electronic and photonic [12]-[14]. Based on the phenomenon that is able to manipulate the addition of nanoparticles SPR detection parameters, the idea of increased SPR performance with the addition of nanoparticles [15] [16]
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