Optical properties of in vitro epidermis and their possible relationship with optical properties of in vivo skin

Abstract

Using a spectrophotometer with an internal integrating sphere, the absorption (μ a) and reduced scattering (μ s′) coefficients of in vitro epidermis were evaluated from reflectance and transmittance measurements. μ a and μ s′ varied from 24 to 0.2 cm −1 and from 32 to 21 cm −1 respectively, on passing from 400 to 800 nm. Moreover, using an external integrating sphere, the reflectance spectrum of in vivo skin was compared with the reflectance spectrum calculated with a Monte Carlo model, in which the mean values of μ a and μ s′ and different anisotropy parameters were used as input data. In vivo results show that the principle of similarity is entirely valid for wavelengths greater than 600 nm and may be considered a good approximation in the 400–600 nm band, and suggest that optical characteristics of in vivo skin may be inferred from reflectance measurements.