Abstract
The dependence of the absorbance and the UV absorption limits of typical binary mobile phases for high-performance liquid chromatography (HPLC) on the volume fraction of modifiers was analyzed. The applicability of the found relationships to the multiparametric optimization of the mobile-phase composition with a generalized objective function using the Microsoft Excel 2000 spreadsheet program was considered by the example of microcolumn HPLC of hydrophobic phenol-like additives.
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