Abstract
A simple optical pH sensor based on immobilization, Dioscorea alata L. anthocyanin methanol extract, onto a pectin–chitosan polyelectrolyte complex (pectin–chitosan PEC), has been successfully fabricated. The optical pH sensor was manufactured as a membrane made of pectin–chitosan PEC and the extracted anthocyanin. This sensor has the highest sensitivity of anthocyanin content at 0.025 mg/L in phosphate buffer and 0.0375 mg/L in citrate buffer. It also has good reproducibility with a relative standard deviation (%RSD) of 7.7%, and gives a stable response at time values greater than 5 min from exposure in a buffer solution, and the sensor can be utilized within five days from its synthesis. This optical pH sensor has been employed to determine saliva pH of people of different ages and showed no significant difference when compared to a potentiometric method.
Highlights
Pectin and chitosan are natural polymers that have been widely used in the food industry, medicine, and the environment [1,2,3,4,5,6]
As a result of these salt-like bridges established between the two kinds of oppositely charged groups, pectin–chitosan polyelectrolyte complex (PEC) membrane become practically insoluble in aqueous medium
The interaction between the two polymers pectin and chitosan will be able to improve the mechanical characteristic of the pectin–chitosan PEC membrane produced [15]
Summary
Pectin and chitosan are natural polymers that have been widely used in the food industry, medicine, and the environment [1,2,3,4,5,6]. A chitosan-pectin PEC membrane has been obtained and used as a matrix to develop an optical pH sensor by immobilizing the anthocyanin-a pH sensitive compound extracted from the Dioscorea alata L. During the construction of our optical pH sensor, the selection of the composition ratio was based on the one that could yield a transparent membrane as opposed to the previous study [18] that was based on the stoichiometric calculation This sensor was utilized to determine the acidity of human saliva and is able to work for a small amount of sample. The usefulness of the sensor described relies on the fact that the examination of saliva is a routine analysis especially for dental and oral health assessment [19,20,21]
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