Abstract
An attempt has been made to apply the concepts of “signal” and “noise” so important in communication theory to the analysis of the performance of photodensitometers used for the quantitative assessment of thin media chromatograms. Very low concentrations of separated substances are considered thus permitting the linearization of the relationships involved. It has been shown that for the best performance under these conditions a highly stabilized light source is essential and that the spectral width of the scanning beam should be the same, or somewhat less than that of the absorbing zone of interest. At low concentrations flying-spot scanning as opposed to fixed-slit scanning is of no real advantage. The double-beam system described in the following paper is vastly superior to any single-beam arrangement.
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