Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides.

Kristina A Malsagova,Andrey F Kozlov,Igor N Kupriyanov,Alexander I Archakov,Ivan D Shumov,Fedor V Tikhonenko,Vladimir P Popov,Tatyana O Pleshakova,Rafael A Galiullin,Anna L Kaysheva,Yuri D Ivanov

doi:10.3390/mi12020147

Abstract

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

Highlights

Autism spectrum disorders (ASDs) are general developmental disorders characterized by persistent deficits in the ability to maintain and initiate social interactions and connections and by limited interests and frequently repetitive behaviors
Monitoring of the quality of silicon nanowire (SOI) structures of silicon nanoribbons (Si-NRs) chips was performed by optical
The spectra were excited with a 514 nm Ar+ laser beam

Summary

Introduction

Autism spectrum disorders (ASDs) are general developmental disorders characterized by persistent deficits in the ability to maintain and initiate social interactions and connections and by limited interests and frequently repetitive behaviors. ASDs develop in childhood and continue into adolescence and adulthood. In most cases, these disorders manifest themselves in the first five years of life. ASDs are often accompanied by other disorders, including epilepsy, depression, anxiety, and attention deficit hyperactivity disorder [1,2,3]. The intelligence level of those experiencing ASDs varies from severe cognitive deficits to high cognitive abilities. ASDs appear due to brain disorders and lead to changes at the metabolomic, transcriptomic, and proteomic levels

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