Optical module for single-shot quantitative phase imaging based on the transport of intensity equation with field of view multiplexing.

Abstract

We present a cost-effective, simple, and robust method that enables single-shot quantitative phase imaging (QPI) based on the transport of intensity equation (TIE) using an add-on optical module that can be assembled into the exit port of any regular microscope. The module integrates a beamsplitter (BS) cube (placed in a non-conventional way) for duplicating the output image onto the digital sensor (field of view - FOV - multiplexing), a Stokes lens (SL) for astigmatism compensation (introduced by the BS cube), and an optical quality glass plate over one of the FOV halves for defocusing generation (needed for single-shot TIE algorithm). Altogether, the system provides two laterally separated intensity images that are simultaneously recorded and slightly defocused one to each other, thus enabling accurate QPI by conventional TIE-based algorithms in a single snapshot. The proposed optical module is first calibrated for defining the configuration providing best QPI performance and, second, experimentally validated by using different phase samples (static and dynamic ones). The proposed configuration might be integrated in a compact three-dimensional (3D) printed module and coupled to any conventional microscope for QPI of dynamic transparent samples.