Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice.

Abstract

The molecular characterization of cancer could have significant clinical benefits, including early diagnosis, making treatment decisions, and monitoring therapeutic response. In this regard, noninvasive assessment of expression/activity of specific molecules in tumors could be vital in managing cancer. Optical probes have demonstrated promise in the molecular imaging of cancer. Here, we have described a method to noninvasively assess the activity of matrix metalloproteinases (MMPs) in human prostate tumors in mice. We used an activatable probe MMPSense™ 750 FAST (MMPSense750) with fluorescent properties in the near-infrared (NIR) range with peak excitation at ~749nm and peak emission ~775nm. These optical properties offer the advantage of a higher depth of detection. This probe has shown immense potential in imaging MMPs activity in deeper tissue with high target-specific signal and low background autofluorescence. Therefore, this probe could be valuable in assessing MMPs activity in primary tumors and metastasis.