Year-end Sale % OFF 
Abstract
We present optical tomography methods for a 3D refractive index reconstruction of weakly scattering objects using LED light sources. We are able to record holograms by minimizing the optical path difference between the signal and reference beams while separating the scattered field from its twin image. We recorded multiple holograms by illuminating the LEDs sequentially and reconstructed the 3D refractive index reconstruction of the sample. The reconstructions show high signal-to-noise ratio in which the effect of speckle artifacts is highly minimized due to the partially incoherent illumination of the LEDs. Results from combining different illumination wavelengths are also described demonstrating higher acquisition speed.
Highlights
Optical Diffraction Tomography (ODT) is an emerging tool for label-free imaging of semi-transparent samples in three-dimensional space [1,2,3,4,5,6,7,8,9,10]
To reconstruct the 3D refractive index (RI) map of semi-transparent samples, a holographic detection is needed to extract the phase of the field after passing through the sample
The experimental setup used in our experiments combined a standard bright field microscope (AmScope T490B-DK 40X-2000X, Irvine, CA, USA) in which an LED ring illumination unit (Adafruit, ID: 1586, 24 LED pixels, bandwidth = 20 nm, New York, NY, USA) replaced the bright-field illumination unit incorporated with the commercial microscope
Summary
Optical Diffraction Tomography (ODT) is an emerging tool for label-free imaging of semi-transparent samples in three-dimensional space [1,2,3,4,5,6,7,8,9,10]. Upatnieks and Leith proposed an “off-axis” holography [12] In this configuration, a small tilt is introduced between the reference arm and the sample arm, which results in shifting in the Fourier domain the “out-of-focus” image with respect to the “in-focus”. In [18], Tian and Waller used LED illumination and demonstrated an iterative reconstruction scheme with a multi-slice forward model to estimate the 3D complex RI distribution by minimizing an error function between the intensity patterns estimated from the forward model. Their approach showed in-focus reconstruction at different depths while taking multiple scattering phenomena into account. We show the effect of adding a different wavelength on the final reconstruction
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
