Optical Clearing of the Gastric Mucosa Using 40%-glucose Solution

Vadim Genin,Ekaterina Lazareva,Sergey Kapralov,Valery Tuchin,Veniamin Grishaev,Alexey Bashkatov,Elina Genina

doi:10.18287/jbpe19.05.030302

Abstract

The kinetics of collimated transmittance of the gastric mucosa under the action of an aqueous 40%-glucose solution was experimentally investigated. Based on the analysis of the transmittance kinetics, the value of the effective diffusion coefficient of glucose in the gastric mucosa was estimated and amounted to (1.59±0.96)×10 -6 cm 2 /s. The permeability coefficient of the mucosa for glucose, calculated using the first Fick diffusion law, was estimated as (2.81±0.90)×10 -5 cm/s. It was shown that the introduction of the glucose solution into the mucosa reduced the light scattering coefficient by approximately 5–10%. The increase in the depth of light penetration was from 5% to 15%, depending on the selected spectral range. The results can be used to develop new methods of diagnosis and treatment of stomach diseases.

Highlights

Non-invasive optical methods for diagnostics and therapy of various diseases are widely used in medicine, due to their safety for the health of patients and low cost [1,2,3,4,5,6,7]
As shown in numerous studies, the scattering properties of biological tissues can be effectively controlled by action of hyperosmotic immersion optical clearing agents (OCAs), which cause diffusion of water from interstitial space and partially replace the interstitial fluid
In the process of replacing water molecules in the interstitial fluid with glucose molecules, we have observed the increase in optical transmittance, i.e., the mucosa transparency, which is due to the matching of refractive indices of the interstitial fluid and the tissue scatterers

Summary

Introduction

Non-invasive optical methods for diagnostics and therapy of various diseases are widely used in medicine, due to their safety for the health of patients and low cost [1,2,3,4,5,6,7]. [8,9,10,11,12,13,14,15]), the scattering properties of biological tissues can be effectively controlled by action of hyperosmotic immersion optical clearing agents (OCAs), which cause diffusion of water from interstitial space and partially replace the interstitial fluid. This method is known in literature as a “tissue optical clearing” technique. The described method of the control of tissue optical characteristics is important both for studying fundamental laws of tissue metabolism and for development of the optical and laser methods of diagnostics, therapy and surgery

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