Abstract

UV-VIS spectroscopic analysis of oligonucleotide DNA exposed to different magnetic fields was performed in order to investigate the relationship between DNA extinction coefficients and optical parameters according to magnetic-field strength. The results with the oligonucleotides adenine-thymine 100 mer (AT-100 DNA) and cytosine-guanine 100 mer (CG-100 DNA) indicate that the magnetic field influences DNA molar extinction coefficients and refractive indexes. The imaginary parts of the refractive index and molar extinction coefficients of the AT-100 and CG-100 DNA decreased after exposure to a magnetic field of 750 mT due to cleavage of the DNA oligonucleotides into smaller segments.

Highlights

  • The effects on biomolecules of exposure to a magnetic field have attracted a lot of attention in recent decades

  • The results show a significant effect on the DNA molar extinction coefficient and optical parameters after in vitro magnetic field exposure above 750 mT

  • The UV-VIS spectrum were measured to evaluate extinction coefficient and refractive indexes of the DNA oligonucleotides to the magnetic fields in controlled condition

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Summary

Introduction

The effects on biomolecules of exposure to a magnetic field have attracted a lot of attention in recent decades. In the broad biomaterials category, DNA strand manipulation by moderate intensity magnetic fields has been a topic a growing interest in biology and other scientific research. Immobilization of DNA strands on small chips, mechanical manipulation of DNA by magnetic fields in a magnetic tweezers device, and magnetic arrangement in liquid crystals are some interesting topics reported to date [4,6,7,8]. It should be pointed out here that DNA oligonucleotides with single strand nucleic acid sequences, due to their high sensitivity to detect small particles and virus (e.g., HIV), have scientific significance, prompting us to select this material for this research [12,13,14,15]. The subject of this work is the spectroscopic analysis of the DNA oligonucleotides adenine-thymine

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