Optical biosensor manufacturing for Chronic Lymphocytic Leukemia biomarker detection with -walled carbon nano tubes-based multi electrodes

Abstract

All around world, cancer is a major factor in mortality and reduced life expectancy. Even while several treatments are being developed to reduce mortality, chronic pain, and to improve quality of life, these cancer medications still depend in some areas. Monitoring the electrostatic interactions based Fermi level fluctuation caused by biological and chemical species adsorption here on semiconductor surface allows for the early detection of Chronic Lymphocytic Leukemia (CLL). The detection of Chronic Lymphocytic Leukemia (CLL) biomarkers is critical for early diagnosis and prognosis of blood cancer patients. Despite the development of various treatments to minimize fatalities and chronic pain, there is still a gap in the adequacy of these cancer medicines. The proposed technique in this paper shows a novel approach to understanding the operation of an optical biosensor by presenting an in-depth investigation of the sensing mechanism. The developed biosensor sensitivity limit of detection (LOD) was determined, and the employment of a laser (635nm wavelength) to produce photocurrent and photoconductive response was assessed by monitoring Fermi level fluctuations, making this biosensor unique. This suggested method presents a thorough analysis of the sensing mechanism and illustrates a novel approach to knowing the operation of a biosensor. By altering its silane binding time, antibody concentration, and antibody binding duration on that surface of MWCNTs, the sensor's performance may be increased. In this study, multi-wall carbon nano tubes are used to create an optical biosensor for the detection of chronic lymphocytic leukaemia (CLL) (MWCNTs). In this study, bio-electrode characteristics were enhanced to produce a functional biosensor. In this study, complementary investigations like Raman, FTIR, and FESEM evaluations were performed. The sensing procedure has been described in terms of the Fermi level fluctuation produced by the charge transfer.