Optical and electrochemical dual detection of β-lactoglobulin based on the methylene blue@copper-based metal-organic framework

Abstract

<italic>β</italic>-lactoglobulin is an effective indicator of allergic protein detection. Herein, we produced a copper-based metal-organic framework coated with methylene blue, to realize the optical and electrochemical dual detection of <italic>β</italic>-lactoglobulin. Methylene blue was successfully encapsulated inside the copper-based metal-organic framework and released after addition of <italic>β</italic>-lactoglobulin. As the concentration of<italic> β</italic>-lactoglobulin increased, the intensity of the ultraviolet absorption band and the response current increased with the increasing concentration of methylene blue released from the copper-based metal-organic framework. The optical detection range is from 0.10 mg/mL to 10 mg/mL, and the detection limit is 0.10 mg/mL. The electrochemical detection range is from 1.0 × 10⁻⁷ mg/mL to 8.0 × 10⁻⁷ mg/mL, the detection limit is 2.0 × 10⁻⁸ mg/mL. The dual detection strategy, with no interfere with each other, played a synergetic role in the quick qualitative and precise quantitative analyses of<italic> β</italic>-lactoglobulin in a wide range of applications.