Opsin-based photopigments expressed in the retina of a South American pit viper,Bothrops atrox(Viperidae)

Abstract

AbstractAlthough much is known about the visual system of vertebrates in general, studies regarding vision in reptiles, and snakes in particular, are scarce. Reptiles display diverse ocular structures, including different types of retinae such as pure cone, mostly rod, or duplex retinas (containing both rods and cones); however, the same five opsin-based photopigments are found in many of these animals. It is thought that ancestral snakes were nocturnal and/or fossorial, and, as such, they have lost two pigments, but retained three visual opsin classes. These are theRH1gene (rod opsin or rhodopsin-like-1) expressed in rods and two cone opsins, namelyLWS(long-wavelength-sensitive) andSWS1(short-wavelength-sensitive-1) genes. Until recently, the study of snake photopigments has been largely ignored. However, its importance has become clear within the past few years as studies reconsider Walls’ transmutation theory, which was first proposed in the 1930s. In this study, the visual pigments ofBothrops atrox(the common lancehead), a South American pit viper, were examined. Specifically, full-lengthRH1andLWSopsin gene sequences were cloned, as well as most of theSWS1opsin gene. These sequences were subsequently used for phylogenetic analysis and to predict the wavelength of maximum absorbance (λmax) for each photopigment. This is the first report to support the potential for rudimentary color vision in a South American viper, specifically a species that is regarded as being nocturnal.