Abstract
Changes in polyamine biosynthesis and elongation of etiolated rice coleoptiles (Oryza sativa L. cv. Taichung Native 1) in response to fusicoccin (FC) and indoleacetic acid (IAA) were investigated. FC stimulated coleoptile elongation at concentrations higher than 1 μM but caused a decrease in the levels of free putrescine, spermidine and sper‐mine, as well as in the activities of arginine decarboxylase (ADC, EC 4.1.1.19) and S‐adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.50). The extent to which FC caused these effects was dependent on its concentration. Treatment with 100 μM IAA also induced coleoptile elongation and resulted in a decrease in free spermidine/sper‐mine and SAMDC activity. However, treatment with IAA resulted in an increase in free putrescine levels and ADC activity. The extent of coleoptile elongation and putrescine accumulation also depended on IAA concentration. α‐Difluoromethylarginine (DFMA), an irreversible inhibitor of ADC. but not α‐difluoromethylornithine (DFMO). an irreversible inhibitor of ODC (EC 4.1.1.17), inhibited the LAA‐stimulated coleoptile elongation and putrescine accumulation. Addition of putrescine could not reverse the effect of DFMA.
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